Mohammad Haroon QURESHI

Araştırma

Anti-metastatic drug target identification

Majority of cancer related deaths occur due to metastasis. However, current regimens of anti-cancer drugs do not have anti-metastatic/anti-invasion drugs. Gain of motility through EMT is the common feature of invading cancer cells and actomyosin based contractility is one of the common denominators underlying motility. Actomyosin cortex provides the link between membrane plasticity and cellular contractility. Analysis of cortical proteome that determines motility behavior in a metastatic cancer cell line is thus pertinent to identify key factors and druggable targets. Metabolic reprograming and bioenergetics associated with the migration and mechanosignaling favors metastasis. My research group is interested in employing multi-omics approaches to study the cortical plasticity and altered metabolism associated with cellular migration and metastasis.

Generation of 3D platforms for studying differential and collective migration

My research interest focuses on leveraging 3D culture models to study cancer cell migration and metastasis. Traditional 2D cell cultures lack the complex architecture and interactions found in vivo, which limits their utility in understanding how cancer cells migrate and invade other tissues. Tissue and tumor dynamics are best represented by fast emerging 3D culture technologies such as those based on the spheroids, organoids, and scaffold-based systems. Using these systems, cancer cells’ migration parameters associated with single cell dissemination or collective cell migration can be studied. I am interested in developing novel approaches for 3D cultures aimed at studying tumor dynamics, mechanotransduction and migration of cancer cells. Live cell imaging and multiomics approaches will be utilized to better understand molecular mechanisms associated with metastasis. 

Seçilmiş Yayınlar

• Qureshi MH, Ozlu N, Bayraktar H. Adaptive tracking algorithm for trajectory analysis of cells and layer-by-layer assessment of motility dynamics. Computers in Biology and Medicine. 2022 Nov 1;150:106193.
   
• Khan SU, Eren GO, Atac N, Onal A, Qureshi MH, Cooper FK, et al. Antibacterial type-II InP/ZnO quantum dots via multimodal reactive oxygen species. Chemical Engineering Journal. 2024 Jan 15;480:148140.
   
• Özkan NE, Yigit BN, Degirmenci BS, Qureshi MH, Yapici GN, Kamacıoglu A, et al. Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis. Journal of Cell Science. 2023 Mar 15;136(6):jcs260266.
   
• Ray S, Bandaria JN, Qureshi MH, Yildiz A, Balci H. G-quadruplex formation in telomeres enhances POT1/TPP1 protection against RPA binding. Proceedings of the National Academy of Sciences. 2014 Feb 25;111(8):2990–5.
   
• Han M, Bahmani Jalali H, Yildiz E, Qureshi MH, Şahin A, Nizamoglu S. Photovoltaic neurointerface based on aluminum antimonide nanocrystals. Communications Materials. 2021 Feb 19;2(1):19.
   
• Akdag M, Yunt ZS, Kamacioglu A, Qureshi MH, Akarlar BA, Ozlu N. Proximal Biotinylation-Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins. J Proteome Res. 2020 Aug 7;19(8):3583–92.
   
• Özlü N, Qureshi MH, Toyoda Y, Renard BY, Mollaoglu G, Özkan NE, et al. Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis. The EMBO Journal. 2015 Jan 14;34(2):251–65.
   
• Qureshi MH, Ray S, Sewell AL, Basu S, Balci H. Replication Protein A Unfolds G-Quadruplex Structures with Varying Degrees of Efficiency. J Phys Chem B. 2012 May 17;116(19):5588–94.
   
• Ray S, Qureshi MH, Malcolm DW, Budhathoki JB, Çelik U, Balci H. RPA-Mediated Unfolding of Systematically Varying G-Quadruplex Structures. Biophysical Journal. 2013 May;104(10):2235–45.